Beijing, Feb 1: In a first, Chinese scientists have used a novel version of the CRISPR gene-editing technology to successfully produce live cows with increased resistance to bovine tuberculosis (TB).
The researchers from Northwest A&F University in Chi used a modified version of the CRISPR system called CRISPR/Cas9n to insert a new TB resistance gene — NRAMP1 — into the genome of bovine foetal fibroblasts — a cell derived from female dairy cows.
“We used a novel version of the CRISPR system to successfully insert a TB resistance gene into the cow genome. We were then able to successfully develop live cows carrying increased resistance to TB,” said led author Yong Zhang.
These cells were then used as donor cells in a process called somatic cell nuclear transfer, where the nucleus of a donor cell carrying the new gene is inserted into an egg cell, known as an ovum, from a female cow.
These ovum were then nurtured in the lab into embryos before being transferred into mother cows for a normal pregncy cycle, in which cows were produced with no off target effects on the animals’ genetics — a common problem when creating transgenic animals using CRISPR.
“Importantly, our method produced no off target effects on the cow genetics meaning the CRISPR technology we employed may be better suited to producing transgenic livestock with purposefully manipulated genetics,” Zhang added. For the study, the team used CRISPR to insert the new gene in 11 calves.
The results revealed that NRAMP1 had successfully integrated into the genetic code at the targeted region in all of the calves. (IANS)